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“高速逆流色谱(HSCCC)分离制备川西獐牙菜中的三种口山酮类化合物”一文在Phytochemical Analysis杂志上发表

发布时间:2012-07-23

Jing Jia(贾静), Tao Chen(陈涛), Ping Wang(王萍),Guichen Chen(陈桂琛),Jinmao You(尤进茂),Yongjun Liu(刘永军),and Yulin Li*(李玉林).Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography, Phytochemical Analysis. 2012 ,23:332-336 (全文链接见附件)

Abstract:

Introduction – Xanthones, the primary constituents of Swertia mussotii, are known to possess a variety of biological activities,including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, antiinflammatory,anti-viral, cardiotonic and hypoglycemic properties. However, high performance, environmentally friendlymethods for isolating and purifying xanthones from S. mussotii are not currently available.

Objective – To develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from S. mussotii using high-speed counter-current chromatography (HSCCC).

Methodology – A solvent system composed of n-hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL/min, a rotation speed of 800 rpm and a temperature of 25℃.

Results – Using the described method, 8mg of methylswertianin, 21mg of swerchirin and 11mg of decussatin with purities of over 98% could be isolated from a 150mg crude sample. They were identified by 1H-NMR and 13 C-NMR analysis.

Conclusion – Three xanthones in Swertia mussotii could be systematically isolated and purified using HSCCC.

paration_of_Methylswertianin,_Swerchirin_and_Decussatin_from_the_Tibetan_Medicinal_Plant_Swertia_Mussotii_Using_High-speed_Counter-current_Chromatography.pdf