“利用高速逆流色谱与中压制备色谱分离桃儿七中三种木质素和一种黄酮类化合物”一文在Phytochemical Analysis上发表

　　王萍,刘永玲,陈涛,徐文华,尤进茂,刘永军,李玉林^*.One-step Separation and Purification of Three Lignans and One Flavonol from Sinopodophyllum emodi by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography, Phytochemical Analysis,2013,24:603-607.IF=2.48 

　　Abstract: 

　　Introduction – Lignans and flavonols are the primary constituents of Sinopodophyllum emodi and have been used as cathartic,anthelmintic,chemotherapeutic and anti-hypertensive compounds. Although these compounds have been isolated, there have been no reports on the separation of 40-demethyl podophyllotoxin, podophyllotoxin, deoxypodophyllotoxin and kaempferol in one step by medium-pressure liquid chromatography (MPLC) and high-speed counter-current chromatography (HSCCC). 

　　Objective – Development of an efficient method for the preparative separation and purification of three lignans and one flavonol from S. emodi. 

　　Methods – The precipitate of crude extracts was first separated by MPLC into four parts, numbered GJ-1, GJ-2, GJ-3 and GJ-4.GJ-1 was separated and purified by HSCCC using a solvent system composed of n-hexane:ethyl acetate:methanol:water (1.75:1.5:1:0.75, v/v/v/v). The purities of the target compounds were assessed using high-performance liquid chromatography (HPLC) and chemical structures were identified by 1H-NMR and 13C-NMR. 

　　Results – The HSCCC and MPLC methods were successfully used for the preparative separation and purification of 40-demethyl podophyllotoxin (8.5 mg, 92.4%), podophyllotoxin (40.1 mg, 92.1%), deoxypodophyllotoxin (4.6 mg, 98.1%), and kaempferol (1.6 mg, 96.7%) from a 100 mg sample. 

　　Conclusion – Three lignans (40-demethyl podophyllotoxin, podophyllotoxin, deoxypodophyllotoxin) and one flavonol (kaempferol) were successfully isolated by HSCCC and MPLC in one step. Copyright © 2013 John Wiley & Sons, Ltd.